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KMID : 1025520090510010045
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Journal of Animal Science and Technology
2009 Volume.51 No. 1 p.45 ~ p.52
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Effects of Increasing Inclusion Levels of Rumen Celluloytic Bacteria Culture on In vivo Ruminal Fermentation Patterns in Hanwoo Heifers
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Park Joong-Kook
Jeong Chan-Sung
Park Do-Yeun
Kim Hyun-Cheol
Lee Seung-Cheol
Kim Chang-Hyun
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Abstract
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This experiment was conducted to observe the effects of anaerobic cellulolytic bacteria culture(Ruminococcus flavefaciens H-20 and Fibrobactor succinogenes H-23) on in vivo ruminal fermentation characteristics in Hanwoo heifers. Four ruminally cannulated Hanwoo heifers(221±7.5kg) receiving a basal diet containing 3kg of mixture hay(tall fescue and ochardgrass) and 2kg of concentrate per day were in a 4×4 Latin square with 21-day periods. Treatments were the basal diet without the culture additive(control), the basal diet plus 50ml/day of bacteria culture of H-20 and H-23(1%), 150ml/day of H-20 and H-23(3%), and 250ml/day of H-20 and H-23(5%). In the whole experimental periods, ruminal pH did not differ between treatments. However, the concentration of ruminal ammonia-N was increased in the 3% treatment relative to control and the 1% treatment at 1hr post-feeding(p<0.05). Avicelase and CMCase(carboxymethyl cellulase) activities in rumen fluid showed no significant difference among treatments. However, xylanase activity was higher in the 5%(119.49, xylose μmol/ml/min) than the 3% treatment(71.02, xylose μmol/ml/min) at 0 hr post-feeding(p<0.05). Concentrations of ruminal total VFA, acetate, propionate and valerate were unaffected by treatments, while butyrate was higher in the 3% treatment (24.48mM) than control(15.71mM) at 1hr post-feeding(p<0.05). Results indicate that minimum 3% inclusion of cellulolytic bacteria cultures improved ruminal fermentation, especially ammonia-N concentration and butyric acid production.
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KEYWORD
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Celluloytic bacteria cultures, Hanwoo heifers, Ruminococcus flavefaciens, Fibrobactor succinogenes
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